Electrochemiluminescence Chip for Methyl-cytosine Determination in Dna
نویسنده
چکیده
This paper reports the first array type chip for DNA methylation assay by electrochemiluminescence (ECL) detection. This was achieved using a small volume (141-nL) sample between two thin film electrodes that enabled us to observe an ECL emission derived from an anti methyl-cytosine antibody with a high signal-to-noise ratio. We performed a single methyl-cytosine measurement in DNA without the conventional bisulfite reaction, PCR amplification or sequencing.
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تاریخ انتشار 2012